高效液相色谱法测定抗病毒胶囊中连翘苷的含量.doc

1 高效液相色谱法测定抗病毒胶囊中连翘苷的含量【关键词】抗病毒胶囊; ,, 连翘苷; ,, 高效液相色谱法摘要:目的建立抗病毒胶囊中连翘苷的含量测定方法。方法采用高效液相色谱法测定该药中连翘苷的含量, 色谱条件: ODS C18 柱( mm × 250 mm,5 μm )为色谱柱, 乙***水( 28∶ 72 )为流动相;流速: ml/min ;检测波长: 230 nm 。结果连翘苷浓度范围在 ~ μ g/ml 之间峰面积与浓度线性关系良好, r= 9, 平均回收率为 % , RS D 为 %; 结论该方法准确、可靠、专属性强,可用于抗病毒胶囊的质量控制。关键词:抗病毒胶囊; 连翘苷; 高效液相色谱法 Determination of Forsythin in Anti-virus Capsule by HPLC Abstract : ObjectiveTo establish a method for the determination of forsythin in Anti-virus 2 in Fructus Forsythiae was determined by HPLC. The chromatographic conditions were : ODS-C18(250 mm × mm,5 μm) as analytic column, acetonitrile-water(28 ∶ 72) as mobile phase,the detection wavelength was set at 230 nm and the flow rate was ml/ linearity of forsythin in the range of ~ μ g/ml was good,r= 9,the average recovery was %,RSD=%.ConclusionThe method is accurate,reliable and specific and can be used for the quality control of Anti-virus Capsule. Key words : Antivirus Capsule ; Forsythin ; HPLC 抗病毒片处方来源于中华人民共和国国家药品监督管理局标准,由板蓝根、石膏、芦根、生地黄、郁金、知母、石菖蒲、广藿香、连翘等九味中药组成。板蓝根、连翘清热凉血, 驱除外感邪毒; 石膏、知母、芦根、生地和郁金清肺热, 凉血热兼解散肌表之热; 广藿香疏散表邪; 石菖蒲主咳逆止气。九味组方治内热感冒、上呼吸道感染等症, 可谓合理得当。本品由抗病毒片改制而成, 对上呼吸道感染、支气管炎、流行性出血性结膜炎、腮腺炎有疗效, 对病毒性流感疗效尤为显著。为控制抗病毒胶囊的质量, 本文采用高效液 3 相色谱法测定抗病毒胶囊中连翘苷的含量, 结果表明: 该方法准确,重现性好,可有效控制抗病毒胶囊的质量。 1 仪器与试药 仪器岛津 LC10ATvp 高效液相色谱仪, SPD10Av p 紫外检测器