梯度密度离心法分离肝癌细胞及其转移性细胞株的建立论文.doc

梯度密度离心法分离肝癌细胞及其转移性细胞株的建立论文.doc梯度密度离心法分离肝癌细胞及其转移性细胞株的建立论文
张萌, 胡少为, 姜桔红, 文剑明
【摘要】【目的】建立新的肝细胞癌细胞系,为肝癌实验研究提供更多纯化癌细胞。【方法】从肝细胞癌患者原发灶和门脉癌栓中获取癌组织,采用Percoll浓度梯度分离癌细胞进行培养,用微环境消化法获取癌细胞克隆并建立细胞系,对培养成功的细胞系进行生长曲线测定、染色体G带核型分析、对比基因组杂交和裸鼠接种成瘤实验。【结果】 (HCC) cell lines and provide more pure liver carcinoma cells for HCC studies.【Methods】The carcinoma tissues the primary lesion and metastatic lesion in portal vein of HCC. The carcinoma cells icro-environmental digestion and cell lines osome karyotype, parative genomic hybridization (CGH) of the established cells planted in nude mice for tumorigenesis.【Results】 20 cases of HCC cells 2 cases of emboli of portal vein. The karyotype shoosomes). A marker chromosome containing 1q and 6p [t(1;6)] arker chromosome containing a long homogeneously staining region (hsr) in H4M cells. The main geic alterations analyzed by CGH ber amplification of 1q, 3q, 5p, 6p, 7q and 8q and loss of 4q,.freelplification of 6p, 7p, 11p, 11q13 and loss of 8p, 9, 13q, 16q in H4M cells. Implantation of H2M cells in nude mice for a month produces typical human hepatocellular carcinoma, but no tumor for H4M cells.【Conclusions】 Percoll density gradient centrifuge and micro-environmental digestion is an effective method for cloning of primary carcinoma cell and cell line establishment. Todels for further screening of oncogenes and tumor suppressor genes in HCC.
Key a; HCC cell lines