技能大赛职业教育主题班会..ppt

绒毛栓孔菌菌丝体在无营养条件下对
偶氮染料刚果红的脱色作用
司静 1 闫志辉 2 崔宝凯 1* 张忠民 3
(1. 北京林业大学微生物研究所 北京 100083)
(2. 内蒙古锡林郭勒盟草原工作站 内蒙古锡林浩特 026000)
(3. 济宁学院 山东济宁 273155)
摘要: 【目的】在无营养条件下,利用白腐真菌绒毛栓孔菌(Trametes pubescens)菌丝体对染料进行脱色可减少试验成本,提高染料处理的实用性。【方法】将该菌株液体培养的菌丝体在无营养条件下对染料进行脱色,并对其中脱色效果较好的偶氮染料刚果红的脱色过程进行分析。在此过程中,测定了该菌株分泌的胞外胞内酶活力,优化影响因子如初始 pH 值、温度、染料浓度和盐度,同时利用气相色谱-质谱联用技术分析无营养条件下偶氮染料刚果红的降解产物。植物毒性试验测定刚果红经绒毛栓孔菌菌丝体脱色前后的毒性变化。【结果】菌丝体对偶氮染料刚果红有较好的脱色效果,在初始 pH 值为 ,温度为 30 °C,染料浓度为 80 mg/L, 盐度为 % (质量体积比)时,150 r/min 转速下培养 7 d 后脱色率可达 %。在此过程中, 菌丝体可被连续使用 2 次,且其所分泌的酶系可降解染料。此外,通过气相色谱-质谱联用分析得到刚果红的降解产物为萘***、联苯***和叠氮萘。植物毒性试验显示在无营养条件下的绒毛栓孔菌菌丝体对染料有明显的脱毒作用。【结论】研究发现绒毛栓孔菌菌丝体在无营养条件下的偶氮染料废水处理中具有广阔的应用前景。
关键词: 绒毛栓孔菌菌丝体,连续添加染料,降解产物分析,植物毒性试验
Decolorization of azo dye Congo Red by Trametes pubescens pellets without addition of nutrition
SI Jing1 YAN Zhi-Hui2 CUI Bao-Kai1* ZHANG Zhong-Min3
(1. Institute of Microbiology, Beijing Forestry University, Beijing 100083, China)
(2. Grassland Station of Xilinguole League, Inner Mongolia, Xilinhot, Inner Mongolia 026000, China) (3. Jining University, Jining, Shandong 273155, China)
Abstract: [Objective] White-rot fungus Trametes pubescens pellets without addition of nutrition treatment, a novel design, was used to lower the running cost as well as to enhance convenient availability for dye decolorization. [Methods] Liquid cultured pellets of T. pubescens were used to
基金项目:教育部新世纪优秀人才支持计划项目(No. NCET-11-0585);中央高校基本科研业务费专项资金资助项目(No. BLYJ201304)
*通讯作者:Tel: 86-10-62336309; : baokaicui2013@
收稿日期:2013-03-06;接受日期:2013-04-12;优先数字出版日期():2013-10-25
司静等: 绒毛栓孔菌菌丝体在无营养条件下对偶氮染料刚果红的脱色作用 219
decolorize various dyes without addition of nutrition and then the removal process of the azo dye Congo Red with higher decolorization rate was analyzed. During the decolorization process, the extracellular and intracellular enzyme activities were monitored and the impact factors such as initial pH value, temperature, dye concentration and salinity were optimized. Moreover, metabolites of the dye Co