南京林业大学.doc

南京林业大学.doc南京林业大学
本科毕业论文
题目:木质纤维稀酸水解液的脱毒技术的探索
学院: 化学工程学院
专业: 生物工程
学号: 080204203
学生姓名: 陈雪松
指导教师: 徐勇
职称: 副教授
二O一二年五月十八日
摘要

酸爆玉米秸秆水解液中含有发酵抑制物,如甲酸、乙酸、糠醛和酚类等,它们会抑制下游发酵菌种的生长和代谢,甚至致死,从而导致原料的大量浪费和生产成本的增加,研究发展相应的脱毒技术十分必要。本论文以典型的阴离子型、阳离子型和非离子型三种聚丙烯酰***材料开展了酸爆玉米秸秆水解液脱毒技术的研究。
以1g/L浓度的三种脱毒材料处理30ml水解液体系考查聚丙烯酰***的最佳用量。其中,聚丙烯酰***阴离子型15ml,阳离子型13ml,非离子型40ml;保持温度相同,根据材料特性,改变pH,加入最佳用量,得出最佳pH值,聚丙烯酰***,,;阳离子型聚丙烯酰***在最佳用量和pH条件下,改变温度,得出其最佳温度为25℃。将脱毒后水解液进行旋转蒸发,用休哈塔假丝酵母发酵产乙醇,通过发酵,发现经CPAM脱毒处理的水解液乙醇得率最高为90%,这一数据表明,阳离子型聚丙烯酰***的脱毒效果最佳,同时从侧面说明了,还有一些未知的物质对发酵存在着抑制作用,而CPAM对其具有良好的脱毒作用,最后可以得出本实验的结论:CPAM是本实验的最佳脱毒材料,反应条件:1g/L溶液13ml对应30ml体系,,反应温度25℃,反应时间2h。
关键词:酸爆玉米秸秆;水解液;脱毒;聚丙烯酰***;休哈塔假丝酵母
Abstract
Steam explosion pretreated corn stover steeped with sulfuric acid s contains fermentation inhibitors, such as formic acid, acetic acid, and furfural and phenolic, etc, they suppress the growth of downstream fermentating strains and metabolism, and even death, leading to a large waste of materials and increasing the cost of production, study the corresponding introduces is necessary. This paper researched the detoxification of steam explosion pretreated with sulfuric acid steeped by the typical anion and cation type and non-ionic three kinds of polyacrylamide materials.
Handling 30 ml hydrolysate system test PAM best dosage with 1 g/L of the concentration of the three virus-eliminated material. Among them, anionic polyacrylamide type 15 ml, cation type 13 ml, non-ionic 40 ml; Keep the same temperature, according to the material properties, the change pH, join the best dosage, obtains the best pH value, anionic polyacrylamide type , the cation type , or so of nonionic hydrogels; Cation type polyacrylamide in the best dosage and pH condition, the temperature change, and obtain the best temperature of 25 ℃. The course after hydrolysis was rotated evaporation, with Hugh ha tower candida ferment produce ethanol, through the fermentation, has found CPAM virus-free hydrolyze