高效液相色谱法测定感冒热毒清合剂中连翘苷的含量.doc

高效液相色谱法测定感冒热毒清合剂中连翘苷的含量.doc高效液相色谱法测定感冒热毒清合剂中连翘苷的含量[ 摘要] 目的建立感冒热毒清合剂中连翘苷的高效液相色谱( HPLC ) 法含量测定方法。方法对处方中连翘进行定性鉴别,并对连翘苷进行 HPLC 法测定, 采用色谱柱资生堂 SHISEIDO CAPCELL PAK MG-C18 柱( mm × 250 mm,5μm) ,流动相乙***- 水( 76∶ 24) ,流速 mL/min ,柱温 30℃,检测波长 277 nm。结果连翘苷在 ~ μ g/mL 范围内呈良好的线性关系( r= ) ,连翘苷的平均回收率为 % 、 % 、 % 。结论本文首次建立了感冒热毒清合剂中连翘苷的 HPLC 含量测定方法,结果准确,重复性好,可作为感冒热毒清合剂的质量评价方法。[ 关键词] 感冒热毒清合剂;连翘苷;高效液相色谱法;含量测定[ 中图分类号] [ 文献标识码]A[ 文章编号] 1673-7210 ( 2015 ) 05(c) -0117-04 [Abstract] Objective To establish a method on content determination of phillyrin in Ganmao-reduqing Mixture by high performance liquid chromatography ( HPLC ). Methods Phillyrin was performed qualitative identification. The content of phillyrin in mixture was determined by HPLC with a SHISEIDO CAPCELL PAK MG-C18 column ( mm× 250 mm,5μm), acetonitrile-water ( 76∶ 24) was used as mobile phase and the flow rate was mL/min. The columm temperature was set at 30℃ and the wavelength of the detector was set at 277 nm. Results The linear range for phillyrin was - μ g/mL and the correlation cofficient was . The average recovery of phillyrin was % , % , % respectively. Conclusion This is the first time to establish a method using HPLC for determination of phillyrin in Ganmao-reduqing Mixture. The method is accurate , reproducible and suitable to be used for quality assessment of Ganmao-reduqing