【doc】tlmv5‘非编码区基因的克隆与序列分析.doc

【doc】TLMV5‘非编码区基因的克隆与序列分析TLMV5‘非编码区基因的克隆与序列分析瑶U(/中华实验和临床病毒学杂志复9至I4鲞蓥塑堂垒P!TLMV5非编码区基因的克隆与序列分析—A4~—ff-!马为民洪龙徐六妹王火生杨桂林7【摘要】目的调查m阴性的非甲一庚型肝炎患者中一likeminivirus(TLMV)感染情况,对TLMV5非编码区(5NCR)部分基因进行分子克隆与序列分析方法采用巢式FCR技术检测53例|rrv阴性的非甲一庚肝炎患者血清TLMvDNA,对PCR产物进行克隆,(%).对其中8株TLMV基因克隆测序,并与TakahashJ报道的TLMv序列(,a}~326931)比较,其核苷酸序列同源性在64%,83%,庚肝炎的关系尚有待进一步研究【主题词】TLMVi聚台酶链反应;分子克隆;序列分析Moleculardo雌andsequencingof5Jnon—coding画?ofTTv—likeminivirus(TLMV)ZHOUBop—,MAWt6nin,eta/Shenzhenlmt/~togy,Sher,zhen518020,China【Abstract】ObjectiveToinvestigatethe刑一likeminiv】ms(TLMV)infectioninChinesepatiemswith11013A——codingreon(5NCR)—PcRfrom53scramsamplesoflionA—Ghepatitispatientswithout刑infectionFCRpl~luctswHeclonedandsequencedResultsTLMVDNAwe|epositivein37of53patients(%).,—026931Jandshowednucleotideidentitybetw啪64%一83%0蚰Theresultsofthisstudyin—dicatedthatTLMVinfectionisctMllnlol:linnegativepatientswithnonA—GhepatitisThe5NCRsequenceofTLMVshowedagreatdegreeofdiversityThepathogenicityofTLMVanditsrelationshipto11o1:1A—Ghepatitisneedtobeeonli~edandfurthersludyisrequited.【Keywords1TTV—likeminivims~Pdynrerasechainreaction;Clone;se?nceanalysis;Noa—A—Ghepatitis1997年l2月Nishizawa