茶叶中EGCG防治大鼠非酒精性脂肪肝的实验研究.doc

茶叶中 EGCG 防治大鼠非酒精性脂肪肝的
实验研究#
严煜钧1,彭彬2,林勇1,2,刘仲华1,2**
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(1. 湖南农业大学茶学教育部重点实验室,长沙 410128;
2. 国家植物功能成分利用工程技术研究中心,长沙 410128)
摘要:以高脂饲料喂养大鼠,建立非酒精性脂肪肝大鼠模型;分别以低剂量(10 mg/kg·BW)、
中剂量(50 mg/kg·BW)、高剂量(100 mg/kg·BW)表没食子儿茶素没食子酸酯(EGCG)
灌胃大鼠,探讨 EGCG 对大鼠非酒精性脂肪肝的防治作用。结果表明, 与模型组相比,EGCG
能显著降低非酒精性脂肪肝大鼠的体重,降低血清中甘油三酯(TG)、总胆固醇(TC)和
低密度脂蛋白-胆固醇(LDL-C)含量,降低丙氨酸氨基转移酶(ALT)和天冬氨酸转氨酶
(AST)活性水平,提高高密度脂蛋白-胆固醇(HDL-C)的含量,改善大鼠肝脏氧化应激
状态。肝脏切片显示,EGCG 能减轻肝脏细胞中脂滴的形成,对非酒精性脂肪肝大鼠肝脂肪
变性具有较强的修复作用。脂质代谢相关基因表达分析显示,EGCG 能够调理脂质代谢。实
验结果表明 EGCG 能有效改善和修复大鼠非酒精性脂肪肝损伤。
关键词:茶叶;表没食子儿茶素没食子酸酯;非酒精性脂肪肝;防治作用
中图分类号:;
Research on Therapeutic Effect of EGCG from Tea against
Non-alcoholic Fatty Liver Disease in Rats
YAN Yujun1, PENG Bin2, LIN Yong1,2, LIU Zhonghua1,2
(1. Key Lab of Tea Science of Education Ministry, Hunan Agricultural University,
ChangSha 410128;
2. National Research Center of Engineering Technology for Utilization of Botanical Functional
Ingredients, ChangSha 410128)
Abstract: In order to investigate the therapeutic effects of EGCG on the non-alcoholic fatty liver
disease (NALFD), experimental groups were given daily oral administration EGCG with low dose
of 10 mg/kg·BW, middle dose of 50 mg/kg·BW and high dose of 100 mg/kg·BW, respectively.
The results showed that EGCG could lower the body weight gains of NAFLD rats, reduce the
concentration of triglyceride