不明原因复发性流产患者绒毛组织中hsa-miR-125a-5p及其靶基因表达的研究.pdf

该【不明原因复发性流产患者绒毛组织中hsa-miR-125a-5p及其靶基因表达的研究 】是由【小泥巴】上传分享，beplayapp体育下载一共【6】页，该beplayapp体育下载可以免费在线阅读，需要了解更多关于【不明原因复发性流产患者绒毛组织中hsa-miR-125a-5p及其靶基因表达的研究 】的内容，可以使用beplayapp体育下载的站内搜索功能，选择自己适合的beplayapp体育下载，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此beplayapp体育下载到您的设备，方便您编辑和打印。:..·106·??????2023?1??32??1?DOI:.1004-????????????????hsa-miR-125a-5p?????????????1,??2,???1,??1*(1.??????????????,К?100081;??????????,К?100080)【??】?????????????(URSA)???????????RNA(miRNA)hsa-miR-125a-5p??????????????2(MMP-2)???,??hsa-miR-125a-5p?URSA??。?????????2020?1??2021?10??К?????????????25?URSA???????(URSA?),??28???????????(???)。????????PCR???????????hsa-miR-125a-5p??????????-2mRNA??;??????????MMP-2?????。??URSA?????hsa-miR-125a-5p?????????????[(±)vs.(±),?<],????-2mRNA[(±)vs.(±)]???????[(±)vs.(±)]????????(?<)。??URSA???????hsa-miR-125a-5p?????,????????MMP-2?????,???????????????、??????????,?????????。【???】?????;??;??RNA;miR-125a-5p;???????2【?????】【?????】A???????????????-???-????-?????????????????????????????????????????????????????????????????????????????????????????????-???1,??????2,?????????-???1,????????1*?1.??????????????????????????????????????????,???????1000812.??????????????????????????????????????????????????,???????100080【????????】?????????:TodetecttheexpressionofdifferentialmicroRNA(miRNA)hsa-miR-125a-5panditspossibletargetgenematrixmetalloproteinase2(MMP-2)invillustissueofpatientswithunexplainedrecurrentspontaneousabortion(URSA),andtoanalyzethecorrelationbetweenhsa-miR-125a-5pandunexplainedrecurrentspontaneousabortion.???????:Twenty-fiveURSApatientswhowerediagnosedandtreatedinBeijingHaidianDistrictMaternal&ChildHealthHospitalfromJanuary2020toOctober2021wereselectedastheobjects(URSAgroup),andtwenty--timePCRwasusedtodetecttherelativeexpressionofhsa-miR-125a-5pandMMP--2proteinlevelwasdetectedbyimmunohistochemicalmethod.???????:Therelativeexpressionofhsa-miR-125a-5pinvillustissueofURSAgroupwassignificantlyhigherthanthatofcontrolgroup[(±)vs.(±),?<],whiletheexpressionlevelsofMMP-2mRNA[(±)vs.(±)]andprotein[(±)vs.(±)]in【????】2022-10-10;【????】2022-11-10【????】???????????Ч???????(2019GJM06)【????】???,?,К??,??,?????,????????????.(*????):..??????2023?1??32??1?·107·paredwithcontrolgroup(?<).???????????:Theabnormalexpressionofhsa-miR-125a-5pinvillustissueofURSApatientsmayaffectthenormalexpressionofitstargetgeneMMP-2,whichmayleadtodecreasedembryoadhesionandimplantationability,impairedtrophoblastinvasion,andleadtorecurrentabortion.????????:Recurrentspontaneousabortion;Villus;miRNA;miR-125a-5p;Matrixmetalloproteinase2(??????????2023,32(01):106-111)?????(recurrentspontaneousabortion,????:????????????、???RSA)????????????????3???????????、?????、??????、???????,????2????????????????????????。??????????,???????????ο????????????,????28?[1]????????????????????。??????3????。RSA????????????、????????、??????、????:??????;?β-HCG???????????、??????。??????????????,???????。RSA??????,??????RSA(unexplained???????????????????、recurrentspontaneousabortion,URSA),??RSA?????、?????、????、??????[2]??。?50%??。?????????????????,??????????????。??,?Ч????????????????????URSA?????,??????????????????。???????????。??????????、?。????????????????????,URSA??1.?Ч??:??????????????????????????????????????,????????。?????????2RNA(miRNAs),????hsa-miR-125a-5p,????,????????,????????-80℃????????????????????;???;????4%???????,???????????????????????2(MMP-2)?、??。?hsa-miR-125a-5p??????(??PCR??????.2???????????hsa-miR-?)。????????MMP-2?hsa-miR-125a-5p125a-5p??:?????,?????miRNeasy????[3]。Ч???????Ч??URSA?MicroKit(QIAGEN,??)??、????????????hsa-miR-125a-5p????????miRNA。????????????Ч??,?,?????????MMP-2???????A260/280≥?????。??Mir-XmiRNA?????????????????????First-StrandSynthesisKit(Takara,?Ч)????,???hsa-miR-125a-5p?URSA?????。miRNA?????cDNA。??TBGreenPremixExTaqTMⅡ(TliRNaseHPlus;Takara,??????Ч)????????PCR,U6??????。?M????PCR??(ThermoFisher,?、???PCR,??PCR????????)????????????2020?1??2021?10????К??:95℃30s;95℃5s、60℃30s,??40???;??????。min1℃95,min1℃60、s5℃95??????????????????????Ч25?URSA???URSA?。?3???,????。?Msoftware-△△Ct????:20?≤??<40?;?β-HCG?????2??,?????????hsa-??????????;B???????,???miR-125a-5p??????。?PCR??????.3?2???;??12???????;???????????MMP-2????????。mRNA????:??Trizol?????????:..·108·??????2023?1??32??1??mRNA,??SMARTMMLVReverseTranscriptase?PCR,??????:95℃30s;95℃5s、60℃(Takara,?Ч)???mRNA?40??,s31。cDNA???????;95℃5s,60℃1min,95℃TM??TBGreenPremixExTaqⅡ(TliRNaseH1min。??3?Ч???????,????。?Plus;Takara,?Ч)????????PCR,?????????,TM-△△Ct??????。??StepOne???2???PCR????MMP-2mRNA???。???(ThermoFisher,??)????????PCR?????????(?1)。????????PCR????????????(5’-3’)???-GCAGTGACGGAAATGGGACAGACGGAAGAAAATReverseGGCTGTTGTCATACTTCTCATGG?6ForwardCTCGCTTCGGCAGCACATATACTReverseACGCTTCACGAATTTGCGTGTChsa-miR-125a-TGTGA4.???????????MMP-2???????????????。?????:?????????????、??????????±???(?珚±?)??,????б?????3%?H2O2????10min;?????????,?)?、?????(?2)。?????Ч??б?(?±?)??????BMI/(kg/m2)????/dURSA?±±±ⅱ剟???±±±ⅱ剟?,)

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